GPKOW: A Protein That Binds RNA and Is Regulated by PKA
Author Information
Author(s): Aksaas Anne Kristin, Larsen Anja CV, Rogne Marie, Rosendal Ken, Kvissel Anne-Katrine, Skålhegg Bjørn Steen
Primary Institution: University of Oslo
Hypothesis
The specificity of the PKA C subunit in regulating pre-mRNA splicing may be mediated indirectly through other nuclear proteins.
Conclusion
GPKOW is an RNA-binding protein that binds RNA in a PKA regulated fashion, suggesting that PKA phosphorylation is involved in regulating RNA processing.
Supporting Evidence
- GPKOW was identified as an interaction partner for the PKA C subunit Cbeta2 through yeast two-hybrid screening.
- GPKOW binds RNA in a PKA sensitive manner as demonstrated by RNA immunoprecipitation.
- Phosphorylation of GPKOW by PKA at specific sites was shown to regulate its RNA-binding ability.
Takeaway
GPKOW is a protein in the nucleus that helps bind RNA, and its ability to do this is controlled by another protein called PKA.
Methodology
Yeast two-hybrid screening and RNA immunoprecipitation were used to identify interactions and binding capabilities.
Limitations
The interaction between GPKOW and PKA Cβ2 was inconsistent, observed in only about 30% of experiments.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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