Gene Expression Changes in ADAM10 Transgenic Mice
Author Information
Author(s): Claudia Prinzen, Dietrich Trümbach, Wolfgang Wurst, Kristina Endres, Rolf Postina, Falk Fahrenholz
Primary Institution: Johannes Gutenberg-University, Institute of Biochemistry, Mainz, Germany
Hypothesis
Does overexpression of ADAM10 in transgenic mice influence gene expression related to Alzheimer's disease?
Conclusion
Overexpression of ADAM10 leads to only moderate changes in gene expression, with a decrease in inflammation markers.
Supporting Evidence
- 355 genes were differentially expressed in ADAM10 transgenic mice compared to wild-type.
- 143 genes were differentially expressed in dnADAM10 mice compared to wild-type.
- ADAM10 overexpression reduced the expression of inflammation markers S100a8 and S100a9.
Takeaway
Scientists studied mice with extra ADAM10 to see how it affects their genes. They found that it didn't change much, but it helped reduce some signs of inflammation.
Methodology
Microarray analysis and real-time RT-PCR were used to assess gene expression in the brains of transgenic mice.
Potential Biases
Potential bias due to the limited genetic backgrounds of the mouse models used.
Limitations
The study focused on adult mice, which may not reflect changes in younger animals or specific brain regions.
Participant Demographics
Transgenic mice aged five months, including both male and female.
Statistical Information
P-Value
0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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