Comparative Study of Bovine Herpesvirus-5 Strains
Author Information
Author(s): Ladelfa María F, Del Médico Zajac María P, Kotsias Fiorella, Delgado Fernando, Muylkens Benoît, Thiry Julien, Thiry Etienne, Romera Sonia A
Primary Institution: Virology Institute, Veterinary and Agricultural Science Research Centre (CICVyA), National Institute of Agricultural Technology (INTA), Argentina
Hypothesis
How do the in vitro and in vivo properties of two bovine herpesvirus-5 strains compare?
Conclusion
Both strains of bovine herpesvirus-5 induce a similar degree of virulence in cattle, despite differences in their in vitro replication capabilities.
Supporting Evidence
- The A663 strain showed lower lytic capacity and cell-to-cell spread compared to the N569 strain.
- Both strains induced similar degrees of pathogenicity in cattle.
- Viral excretion profiles were similar for both strains in infected calves.
- Histopathological examination revealed severe lesions in the CNS associated with both strains.
Takeaway
This study looked at two types of a virus that makes cows sick and found that while one type doesn't grow as well in the lab, both can make cows equally sick.
Methodology
The study involved in vitro assays for viral growth and an experimental infection of calves with both strains to assess viral excretion and clinical signs.
Limitations
The study's findings may not fully represent the behavior of field strains due to the use of reference strains adapted to cell culture.
Participant Demographics
Ten 3-month-old calves were used in the study.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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