Betanodavirus Induces Cell Death in Fish Cells Through Oxidative Stress
Author Information
Author(s): Chang Chih-Wei, Su Yu-Chin, Her Guor-Mour, Ken Chuian-Fu, Hong Jiann-Ruey
Primary Institution: National Cheng Kung University
Hypothesis
The role of oxidative stress in the pathogenesis of RNA nervous necrosis virus infection is still unknown.
Conclusion
RGNNV infection induces oxidative stress that leads to cell death, but this can be mitigated by antioxidants.
Supporting Evidence
- RGNNV infection induced ROS production at 24, 48, and 72 hours post-infection.
- Antioxidants reduced ROS production and improved cell viability.
- Zebrafish catalase overexpression significantly enhanced cell survival.
Takeaway
When fish cells get infected by a virus, they can get really stressed out and die, but giving them special medicine can help them feel better.
Methodology
The study used GF-1 fish cells infected with RGNNV to assess ROS production and cell viability with various treatments.
Limitations
The study did not explore the long-term effects of antioxidant treatment beyond 72 hours post-infection.
Participant Demographics
GF-1 fish cells were used in the experiments.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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