Neocentromeres Form Efficiently at Multiple Possible Loci in Candida albicans
Author Information
Author(s): Marshall Owen J., Choo K. H. Andy
Primary Institution: Murdoch Childrens Research Institute
Hypothesis
What are the mechanisms of neocentromere formation in Candida albicans?
Conclusion
The study demonstrates that neocentromeres can form at multiple locations along Chromosome V in Candida albicans without loss of mitotic stability.
Supporting Evidence
- Neocentromeres formed in all transformants at multiple locations along Chromosome V.
- Proximal neocentromeres formed near the original centromere, while distal neocentromeres formed at other locations.
- Transformants with distal neocentromeres showed no loss of mitotic stability.
- Transformants with proximal neocentromeres had shifted neocentromeres that silenced URA3 expression.
Takeaway
This study shows that new centromeres can pop up in different places on a chromosome, which is surprising and important for understanding how cells work.
Methodology
The authors removed the centromeric DNA on Chromosome V and observed the formation of neocentromeres using chromatin immunoprecipitation.
Limitations
Only a few distal neocentromeres were analyzed, making it difficult to identify potential hotspots for neocentromere formation.
Digital Object Identifier (DOI)
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