Study of Gene Expression in Ichthyophthirius multifiliis
Author Information
Author(s): Jason W Abernathy, Peng Xu, Ping Li, De-Hai Xu, Huseyin Kucuktas, Phillip Klesius, Covadonga Arias, Zhanjiang Liu
Primary Institution: Auburn University
Hypothesis
To generate expressed sequence tags (ESTs) for the development of a microarray platform to analyze gene expression in Ichthyophthirius multifiliis.
Conclusion
The generated ESTs represent a significant portion of the Ich transcriptome and provide a basis for future gene expression studies.
Supporting Evidence
- Sequenced 10,368 EST clones with a 94.2% success rate.
- Generated 8,432 high quality sequences.
- Identified 4,706 unique sequences from the EST analysis.
Takeaway
Scientists studied a fish parasite by looking at its genes to understand how it causes disease, which can help in fighting it.
Methodology
Sequenced 10,368 EST clones from pooled samples of different life-cycle stages of the parasite.
Limitations
Pooling samples limited the ability to profile gene expression across different developmental stages.
Digital Object Identifier (DOI)
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