How VAMP7 is Sorted into Endocytic Vesicles
Author Information
Author(s): Pryor Paul R., Jackson Lauren, Gray Sally R., Edeling Melissa A., Thompson Amanda, Sanderson Christopher M., Evans Philip R., Owen David J., Luzio J. Paul
Primary Institution: University of Cambridge
Hypothesis
The study investigates the mechanism by which the SNARE VAMP7 is sorted into clathrin-coated vesicles by the ArfGAP Hrb.
Conclusion
Hrb acts as a specific adaptor for the clathrin-mediated endocytosis of VAMP7, facilitating its retrieval from the cell surface.
Supporting Evidence
- Hrb was identified as a binding partner of VAMP7 through yeast two-hybrid screening.
- Depletion of Hrb resulted in increased levels of VAMP7 on the cell surface.
- Mutations in the VAMP7 longin domain disrupted its interaction with Hrb.
Takeaway
This study shows that a protein called Hrb helps another protein, VAMP7, get into tiny bubbles that transport it inside the cell, like a delivery truck picking up packages.
Methodology
The study used yeast two-hybrid screening, pull-down assays, and immunofluorescence microscopy to investigate protein interactions and localization.
Digital Object Identifier (DOI)
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