Accumulation and dissemination of prion protein in experimental sheep scrapie in the natural host
2009

Study of Prion Protein in Sheep Scrapie

Sample size: 25 publication 10 minutes Evidence: high

Author Information

Author(s): Ryder Stephen J, Dexter Glenda E, Heasman Lindsay, Warner Richard, Moore S Jo

Primary Institution: Department of Pathology, Veterinary Laboratories Agency

Hypothesis

How do scrapie prions accumulate and spread in sheep?

Conclusion

The study found that prion accumulation in sheep occurs through lymphatic and haematogenous spread, with a notably short incubation period.

Supporting Evidence

  • All lambs infected at 14 days developed clinical signs after an average of 6 months.
  • Prion uptake was first detected in the Peyer's patch of the distal ileum.
  • Neuroinvasion occurred in the enteric nervous system before the central nervous system.

Takeaway

Researchers gave young sheep a prion infection and found that the disease spread quickly through their bodies, similar to how it happens in nature.

Methodology

Lambs were orally infected with scrapie prions and monitored for disease progression and prion accumulation in tissues.

Limitations

The study primarily used a specific genetic type of sheep, which may limit the generalizability of the findings.

Participant Demographics

Lambs aged 14 days, homozygous for the VRQ allele of the PrP gene.

Digital Object Identifier (DOI)

10.1186/1746-6148-5-9

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