EphB6 Receptor Modulates Micro RNA Profile of Breast Carcinoma Cells
2011
EphB6 Receptor and Micro RNA Changes in Breast Cancer Cells
Sample size: 6
publication
Evidence: moderate
Author Information
Author(s): Bhushan Lokesh Kandpal, Raj P. Navarro
Primary Institution: Western University of Health Sciences
Hypothesis
EphB6 transfection influences the protein profile by modulating the abundance of miRNA complement in breast carcinoma cells.
Conclusion
EphB6 transfection alters the micro RNA profile in breast carcinoma cells, leading to decreased invasiveness.
Supporting Evidence
- EphB6 is a signature molecule for invasive breast carcinoma cells.
- Transfection of EphB6 leads to decreased invasiveness of MDA-MB-231 cells.
- Significantly altered miRNAs included miR-16, miR-23a, miR-24, and miR-100.
Takeaway
When a specific protein called EphB6 is added to breast cancer cells, it changes the types of tiny molecules (miRNAs) in the cells, which helps the cells become less invasive.
Methodology
The study used RNA hybridization and real-time qPCR to analyze miRNA profiles in vector-transfected and EphB6-transfected MDA-MB-231 cells.
Statistical Information
P-Value
<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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