TTRAP and Its Role in TGF-β Signaling
Author Information
Author(s): Várady György, Sarkadi Balázs, Fátyol Károly
Primary Institution: Membrane Research Group, Hungarian Academy of Sciences, Budapest, Hungary
Hypothesis
TTRAP is a novel component of the non-canonical TRAF6-TAK1 TGF-β signaling pathway.
Conclusion
TTRAP is an important component of Smad-independent non-canonical TGF-β induced signaling responses, particularly in the p38 kinase cascade and the NF-κB pathway.
Supporting Evidence
- TTRAP associates with TGF-β receptors and components of the TRAF6-TAK1 signaling module.
- TTRAP enhances the E3 ubiquitin ligase activity of TRAF6.
- Overexpression of TTRAP activates p38 MAP kinase.
- Knockdown of TTRAP abolishes TGF-β induced rapid phosphorylation of p38.
Takeaway
TTRAP helps cells respond to a signal called TGF-β, which can tell them to grow or die. When TTRAP is present, it can make cells more likely to die when they receive this signal.
Methodology
The study involved cell culture, transfection, co-immunoprecipitation, and various assays to analyze protein interactions and cellular responses.
Digital Object Identifier (DOI)
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