A fiber-modified adenoviral vector interacts with immunoevasion molecules of the B7 family at the surface of murine leukemia cells derived from dormant tumors
2011

Adenoviral Vector Interacts with Immune Evasion Molecules in Leukemia Cells

publication 10 minutes Evidence: moderate

Author Information

Author(s): Grellier Elodie, Lécolle Katia, Rogée Sophie, Couturier Cyril, D'Halluin Jean-Claude, Hong Saw-See, Fender Pascal, Boulanger Pierre, Quesnel Bruno, Colin Morvane

Primary Institution: INSERM UMR 837, Lille, France

Hypothesis

The study investigates whether the B7 family molecules on dormant leukemia cells influence the permissiveness of these cells to an adenoviral vector.

Conclusion

The study found that the interaction between the adenoviral vector Ad5FB4 and B7 family molecules on leukemia cells could provide new opportunities for cancer therapy.

Supporting Evidence

  • Leukemia cells that overexpress B7-H1 and B7.1 were more permissive to the adenoviral vector.
  • B7.1 was found to be involved in the attachment of the adenoviral vector to the cells.
  • B7-H1 played a role in the internalization of the adenoviral vector.
  • Ad5FB4 penton capsomeres interfered with the formation of B7.1/B7-H1 heterodimers.

Takeaway

This study shows that certain leukemia cells can be targeted by a modified virus because they have special proteins on their surface that help the virus enter the cells.

Methodology

The study used a mouse model of tumor dormancy and various cell lines to assess the interaction between the adenoviral vector and B7 family molecules through binding assays and flow cytometry.

Limitations

The study primarily focuses on murine models, which may not fully represent human responses.

Participant Demographics

The study involved murine leukemia cell lines derived from dormant tumors.

Statistical Information

P-Value

p<0.01

Statistical Significance

p<0.01

Digital Object Identifier (DOI)

10.1186/1476-4598-10-105

Want to read the original?

Access the complete publication on the publisher's website

View Original Publication