How Actin Dynamics Affect Retinoid Receptor Function in T Cells
Author Information
Author(s): Ishaq Mohammad, Lin Bor-Ruei, Bosche Marjorie, Zheng Xin, Yang Jun, Huang Dawei, Lempicki Richard A, Natarajan Ven
Primary Institution: Laboratory of Molecular Cell Biology, SAIC-Frederick, National Cancer Institute, Frederick, MD, USA
Hypothesis
Actin dynamics and the LIMK1-mediated CFL1 pathway play a critical role in retinoid receptor function during T cell activation.
Conclusion
The study identifies that LIMK1-mediated CFL1 pathway and actin dynamics are crucial for maintaining retinoid receptor activity in T lymphocytes.
Supporting Evidence
- Agents that disturb F-actin assembly or disassembly attenuated receptor-mediated transcription.
- Overexpression or siRNA-induced knockdown of cofilin-1 induced the loss of receptor function.
- Nef induced phosphorylation of CFL1 and inhibited retinoid-receptor mediated reporter activity.
Takeaway
This study shows that the way actin behaves in cells is really important for how well certain receptors work, especially in T cells, which are part of our immune system.
Methodology
The study involved transfecting Jurkat T cells with various plasmids and measuring luciferase activity to assess retinoid receptor-mediated transcription.
Digital Object Identifier (DOI)
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