New On-Axis Multimode Spectrometer for Macromolecular Crystallography
Author Information
Author(s): Owen Robin L., Pearson Arwen R., Meents Alke, Boehler Pirmin, Thominet Vincent, Schulze-Briese Clemens
Primary Institution: Swiss Light Source, Paul Scherrer Institute
Conclusion
The new on-axis multimode spectrometer enhances the ability to study macromolecular structures and their radiation-induced changes.
Supporting Evidence
- The spectrometer allows for the collection of both UV-Vis absorption and Raman spectra.
- It provides complementary information to X-ray diffraction data.
- The on-axis design reduces the need for time-consuming alignment procedures.
- It enables the monitoring of radiation damage in protein crystals.
Takeaway
Scientists built a new tool that helps them see tiny details in proteins and other big molecules, even when they get damaged by light.
Methodology
The study involved the integration of a new spectrometer into the beamline environment, allowing simultaneous UV-Vis absorption and Raman spectroscopy.
Limitations
The spectrometer is not yet permanently installed and requires further commissioning for optimal use with protein crystals.
Digital Object Identifier (DOI)
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