The SOCS2 Ubiquitin Ligase Complex Regulates Growth Hormone Receptor Levels
2011
How SOCS2 Regulates Growth Hormone Receptor Levels
publication
Evidence: high
Author Information
Author(s): Vesterlund Mattias, Zadjali Fahad, Persson Torbjörn, Nielsen Michael Lund, Kessler Benedikt M., Norstedt Gunnar, Flores-Morales Amilcar
Primary Institution: Karolinska Institutet, Stockholm, Sweden
Hypothesis
SOCS2 regulates GHR turnover through proteasomal degradation.
Conclusion
SOCS2 acts as a negative regulator of GHR signaling by promoting its degradation.
Supporting Evidence
- SOCS2 deficient mice show a 40% increase in body size due to enhanced postnatal growth.
- SOCS2 is part of a multimeric complex with intrinsic ubiquitin ligase activity.
- SOCS2 regulates cellular GHR levels through direct ubiquitination.
- Proteasome inhibitors counteract the reducing effect of SOCS2 on receptor levels.
Takeaway
SOCS2 helps control how much growth hormone receptor is in our cells, which is important for growth and metabolism.
Methodology
The study used mutagenesis and co-transfection experiments in HEK293T cells to analyze the effects of SOCS2 on GHR levels.
Digital Object Identifier (DOI)
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