Complete Genomic Sequence of Bacteriophage Felix O1
Author Information
Author(s): Whichard Jean M., Weigt Lee A., Borris Douglas J., Li Ling Ling, Zhang Qing, Kapur Vivek, Pierson F. William, Lingohr Erika J., She Yi-Min, Kropinski Andrew M., Sriranganathan Nammalwar
Primary Institution: Centers for Disease Control and Prevention
Hypothesis
Felix O1 is proposed as a therapeutic or decontaminating agent due to its ability to infect almost all Salmonella isolates.
Conclusion
The study provides a detailed analysis of the genome, proteome, and transcriptome of bacteriophage Felix O1.
Supporting Evidence
- Bacteriophage O1 is a Myoviridae A1 group member used historically for identifying Salmonella.
- The genome of Felix O1 is a single linear DNA molecule of approximately 86,155 base pairs.
- Felix O1 lyses 98.2% of Salmonella and less than 1.4% of other Enterobacteriaceae.
- Proteomic analysis identified multiple structural proteins associated with the phage.
- Felix O1 has been proposed as a therapeutic agent due to its specificity for Salmonella.
Takeaway
Bacteriophage Felix O1 is a virus that can kill most Salmonella bacteria, and scientists have studied its DNA to understand how it works.
Methodology
The genome was sequenced using 1,493 sequencing reactions resulting in >7-fold coverage, and the analysis included genome assembly, open reading frame identification, and proteomic analysis.
Limitations
The study does not address the potential for resistance development in Salmonella against Felix O1.
Digital Object Identifier (DOI)
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