A Novel Endo-Hydrogenase Activity Recycles Hydrogen Produced by Nitrogen Fixation
2009

Endo-Hydrogenase Activity in Azorhizobium caulinodans

publication Evidence: moderate

Author Information

Author(s): Gordon Ng, Tom Curtis, Angela Park, Ludwig Zenad Lounis, Robert A. Ludwig

Primary Institution: University of California Santa Cruz

Hypothesis

The study investigates the role of a novel endo-hydrogenase in recycling hydrogen produced by nitrogen fixation in Azorhizobium caulinodans.

Conclusion

The study concludes that the Hyq endo-hydrogenase is essential for recycling endogenous hydrogen produced during nitrogen fixation in A. caulinodans.

Supporting Evidence

  • A. caulinodans has two types of hydrogenases: one for recycling hydrogen and another for using external hydrogen.
  • The Hyq endo-hydrogenase is specifically induced in nitrogen-fixing conditions.
  • Growth experiments showed that the Hyq endo-hydrogenase is crucial for efficient growth in nitrogen-limited environments.

Takeaway

This study found that a special enzyme helps bacteria recycle hydrogen they produce while fixing nitrogen, which could be useful for renewable energy.

Methodology

The researchers used genetic mutations and growth experiments to analyze the role of the Hyq endo-hydrogenase in A. caulinodans.

Digital Object Identifier (DOI)

10.1371/journal.pone.0004695

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