Modulation of human uterine smooth muscle cell collagen contractility by thrombin, Y-27632, TNF alpha and indomethacin
2009

Effects of Thrombin and Other Agents on Uterine Muscle Cell Contraction

publication 10 minutes Evidence: moderate

Author Information

Author(s): Joan Fitzgibbon, John J. Morrison, Terry J. Smith, Margaret O'Brien

Primary Institution: National University of Ireland Galway

Hypothesis

The study aims to investigate the effects of various uterine contractility agents on human uterine smooth muscle cells.

Conclusion

Thrombin and TNF alpha increase collagen contractility in uterine smooth muscle cells, while indomethacin inhibits it.

Supporting Evidence

  • TNF alpha increased collagen contractility in human uterine smooth muscle cells.
  • Indomethacin significantly inhibited contraction in the study.
  • Thrombin augmented contractility in both primary and immortalized myometrial smooth muscle cells.

Takeaway

This study looked at how different substances affect the way uterine muscle cells contract, which is important for understanding labor.

Methodology

The study used a collagen gel contraction assay with human primary uterine smooth muscle cells and immortalized myometrial smooth muscle cells to measure contractility.

Limitations

The in vitro model may not fully replicate in vivo conditions and has limitations in generalizability.

Statistical Information

P-Value

p<0.001

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1477-7827-7-2

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