SpliceCenter: A suite of web-based bioinformatic applications for evaluating the impact of alternative splicing on RT-PCR, RNAi, microarray, and peptide-based studies
2008

SpliceCenter: Web Tools for Analyzing Alternative Splicing

Sample size: 50766 publication Evidence: moderate

Author Information

Author(s): Ryan Michael C, Zeeberg Barry R, Caplen Natasha J, Cleland James A, Kahn Ari B, Liu Hongfang, Weinstein John N

Primary Institution: National Cancer Institute, National Institutes of Health

Hypothesis

How does alternative splicing impact the interpretation of biological assays?

Conclusion

SpliceCenter provides unique, user-friendly applications for assessing the impact of transcript variation on various biological assays.

Supporting Evidence

  • SpliceCenter tools provide visualizations of a gene's alternative transcripts.
  • 59% of shRNAs in a large-scale library target all known splice variants.
  • The tools are designed for both bench biologists and bioinformaticists.

Takeaway

SpliceCenter is a set of online tools that helps scientists understand how different versions of genes can affect their experiments.

Methodology

Development of web-based applications for analyzing the impact of splice variation on various biological assays.

Limitations

The tools may not cover all possible splice variants and are limited to the data available in the databases used.

Digital Object Identifier (DOI)

10.1186/1471-2105-9-313

Want to read the original?

Access the complete publication on the publisher's website

View Original Publication