Inhibition of Akt Activity in Oral Cancer Cells
Author Information
Author(s): Hong Kyoung-Ok, Kim Ji-Hong, Hong Ji-Soo, Yoon Hye-Jung, Lee Jae-Il, Hong Sam-Pyo, Hong Seong-Doo
Primary Institution: Seoul National University
Hypothesis
Does inhibiting Akt activity restore E-cadherin expression and induce mesenchymal-to-epithelial reverting transition in oral squamous cell carcinoma cells?
Conclusion
Akt inhibition can induce the mesenchymal-to-epithelial reverting transition in oral cancer cells by decreasing NF-κB signaling and downregulating Snail and Twist.
Supporting Evidence
- Akt inhibition led to downregulation of Snail and Twist expression.
- PIA treatment induced the expression of E-cadherin and β-catenin.
- Inhibition of Akt activity reduced tumor cell migration in KB and KOSCC-25B cells.
Takeaway
When scientists blocked a specific protein called Akt in certain cancer cells, it helped the cells go back to a more normal state, which could help treat cancer.
Methodology
The study used various assays including RT-PCR, immunoblotting, and in vitro migration assays to assess the effects of Akt inhibition on cancer cell behavior.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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