U2transLUC: A New Assay for Screening FOXO Modulators
Author Information
Author(s): Zanella Fabian, Rosado Aranzazú, Garcia Beatriz, Carnero Amancio, Link Wolfgang
Primary Institution: Centro Nacional de Investigaciones Oncologicas (CNIO)
Hypothesis
Can a multiplexed assay system effectively screen for FOXO modulators?
Conclusion
The U2transLUC assay is a robust method for identifying small molecules that affect FOXO signaling.
Supporting Evidence
- The U2transLUC system allows simultaneous monitoring of FOXO translocation and transcriptional activity.
- Chemical probes were used to identify compounds that affect FOXO signaling.
- The assay can be automated for high-throughput screening.
Takeaway
This study created a new test that helps scientists find drugs that can change how FOXO proteins work in cells.
Methodology
The study developed a stable cell line expressing a GFP-tagged FOXO transcription factor and a luciferase reporter gene to measure FOXO activity.
Limitations
The assay may not account for all factors influencing FOXO activity in different cellular contexts.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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