Enhancing Single Molecule Imaging in Optofluidics and Microfluidics
2011

Enhancing Single Molecule Imaging in Optofluidics and Microfluidics

publication Evidence: moderate

Author Information

Author(s): Vasdekis Andreas E., Laporte Gregoire P.J.

Primary Institution: Ecole Polytechnique Fédérale de Lausanne (EPFL)

Hypothesis

How can the signal to noise ratio (SNR) be enhanced in optofluidics and microfluidics for single molecule imaging?

Conclusion

The study reviews various methods to enhance the signal to noise ratio during single molecule imaging in optofluidics and microfluidics.

Supporting Evidence

  • Single molecule imaging has grown due to its ability to reveal heterogeneities at high resolutions.
  • Microfluidics allows for reduced reagent consumption and highly multiplexed studies.
  • Optofluidics combines integrated optics with microfluidics to enhance signal detection.

Takeaway

This study looks at ways to make pictures of tiny things, like single molecules, clearer by reducing background noise and boosting the signal.

Methodology

The paper reviews various techniques and strategies for enhancing the signal to noise ratio in single molecule imaging using optofluidics and microfluidics.

Limitations

There is no single ideal procedure applicable to all types of single molecule experiments.

Digital Object Identifier (DOI)

10.3390/ijms12085135

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