Identification of a Variable Number of Tandem Repeats Polymorphism and Characterization of LEF-1 Response Elements in the Promoter of the IDO1 Gene
2011

Study of a Genetic Variation in the IDO1 Gene and Its Impact on Tryptophan Levels

Sample size: 94 publication 10 minutes Evidence: moderate

Author Information

Author(s): Soichot Marion, Hennart Benjamin, Al Saabi Alaa, Leloire Audrey, Froguel Philippe, Levy-Marchal Claire, Poulain-Godefroy Odile, Allorge Delphine

Primary Institution: Université Lille-Nord de France

Hypothesis

The study investigates the association between a VNTR polymorphism in the IDO1 promoter and serum tryptophan concentrations.

Conclusion

The VNTR polymorphism in the IDO1 promoter is associated with lower serum tryptophan concentrations in females, suggesting a role in IDO1 expression variability.

Supporting Evidence

  • A VNTR polymorphism was identified in the IDO1 promoter that correlates with serum tryptophan concentration.
  • The study involved 94 healthy Caucasian subjects to assess the impact of the VNTR on IDO activity.
  • Significant differences in serum tryptophan levels were observed between males and females.
  • Functional analyses revealed novel cis-acting elements in the IDO1 promoter.

Takeaway

This study found a genetic change in a gene that helps control tryptophan levels, which is important for mood and immune function, especially in women.

Methodology

PCR-sequencing strategy was used to identify a VNTR polymorphism in the IDO1 promoter from DNA samples of healthy Caucasians.

Limitations

The study primarily focused on a specific population (Caucasians), which may limit the generalizability of the findings.

Participant Demographics

Healthy Caucasian individuals, with 47 males and 47 females in the study.

Statistical Information

P-Value

0.0006

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0025470

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