Automated Preparation of Biological Samples for Amino-Acid Analysis
Author Information
Author(s): D. C. Turnell, J. D. H. Cooper
Primary Institution: Department of Biochemistry, Coventry and Warwickshire Hospital
Hypothesis
Can dialysis be effectively used for the deproteinization of serum samples in automated amino-acid analysis?
Conclusion
The study demonstrates that combining dialysis with an automated derivatization system can effectively prepare serum samples for amino-acid analysis.
Supporting Evidence
- The automated system can perform sampling, dialysis, derivatization, and injection onto the HPLC in only 135 seconds.
- The mean analytical recovery of amino acids was found to be 101% with a standard deviation of 2.3%.
- Using a double lumen probe maximizes the load volume to dead volume ratio in the system.
Takeaway
This study shows a way to automate the process of preparing blood samples to check for amino acids, making it easier and faster.
Methodology
The study utilized an automated system combining dialysis and pre-column derivatization for amino-acid analysis via HPLC.
Limitations
The method may not achieve maximum recovery of amino acids due to the 60-second dialysis time used.
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