Interactions between Casein Kinase Iε (CKIε) and Two Substrates from Disparate Signaling Pathways Reveal Mechanisms for Substrate-Kinase Specificity
2009
How CKIε Chooses Its Targets
publication
Evidence: high
Author Information
Author(s): Caroline Lund Dahlberg, Elizabeth Z. Nguyen, David Goodlett, David Kimelman
Primary Institution: University of Washington
Hypothesis
How do substrates recognize differences between CKI isoforms?
Conclusion
CKIε's interactions with Disheveled and Period reveal mechanisms for substrate specificity.
Supporting Evidence
- CKIε binds to and phosphorylates Disheveled and Period.
- Two key residues in CKIα prevent substrate binding.
- CKIε's C-terminus stabilizes interactions with its substrates.
- Autophosphorylation of CKIε inhibits substrate binding.
Takeaway
This study shows that CKIε binds to specific proteins in a unique way, which helps it know which proteins to work with.
Methodology
The study used GST-pull-down assays and mass spectrometry to analyze protein interactions.
Digital Object Identifier (DOI)
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