Accurate Detection of Recombinant Breakpoints in Whole-Genome Alignments
2009

Detecting Breakpoints in Whole-Genome Alignments

publication Evidence: high

Author Information

Author(s): Oscar Westesson, Ian Holmes

Primary Institution: University of California Berkeley

Hypothesis

We propose a novel method for detecting sites of molecular recombination in multiple alignments.

Conclusion

Our method accurately detects recombinant regions and breakpoints in genomic sequences, outperforming previous methods.

Supporting Evidence

  • We confirm the breakpoint predictions of previous studies and offer novel predictions.
  • Our method allows for the analysis of fast-evolving pathogen sequences.
  • We demonstrate improved precision and flexibility compared to other programs.

Takeaway

This study introduces a new way to find where genes mix together in viruses and bacteria, helping scientists understand how they evolve.

Methodology

We developed a probabilistic approach using a combined algorithm for estimating tree structure and hidden Markov model parameters to detect changes in phylogenetic tree topology.

Limitations

The method may struggle with high diversity or very short recombinant regions.

Digital Object Identifier (DOI)

10.1371/journal.pcbi.1000318

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