Proteomic Response of Histoplasma capsulatum to Low Iron Levels
Author Information
Author(s): Michael S Winters, Daniel S Spellman, Qilin Chan, Francisco J Gomez, Margarita Hernandez, Brittany Catron, Alan G Smulian, Thomas A Neubert, George S Deepe Jr
Primary Institution: University of Cincinnati College of Medicine
Hypothesis
How does Histoplasma capsulatum respond at the protein level to decreased iron availability?
Conclusion
The study identified 42 proteins in Histoplasma capsulatum that are significantly modulated in response to low iron levels, indicating a shift in metabolism to cope with iron scarcity.
Supporting Evidence
- 42 proteins were identified as significantly modulated when iron concentrations were lowered.
- The study created a bioinformatics platform for analyzing the proteome of Histoplasma capsulatum.
- Mass spectrometry revealed proteins involved in glycolysis and the TCA cycle were affected by iron levels.
- Specific proteins like HSP 70 were less abundant in iron-poor conditions.
Takeaway
When Histoplasma capsulatum has less iron, it changes the proteins it makes to survive better in tough conditions.
Methodology
The study used 2D gel electrophoresis and mass spectrometry to analyze protein changes in Histoplasma capsulatum under low iron conditions.
Limitations
The study does not address the long-term effects of iron deprivation on Histoplasma capsulatum.
Statistical Information
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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