Regulation of primate lentiviral RNA dimerization by structural entrapment
2008
Regulation of Primate Lentiviral RNA Dimerization
publication
Evidence: moderate
Author Information
Author(s): Baig Tayyba T, Strong Christy L, Lodmell J Stephen, Lanchy Jean-Marc
Primary Institution: The University of Montana
Hypothesis
Do common mechanisms of SL1 regulation exist among divergent lentiviral leader RNAs?
Conclusion
All three lentiviral RNAs tested in this study showed a local regulation of dimerization through the stabilization of SL1.
Supporting Evidence
- SL1 usage may be down-regulated by long-range interactions involving sequences between SL1 and the first codons of the gag gene.
- Truncation analysis revealed that tight dimerization of the three lentiviral leader RNAs is modulated by interactions of nucleotides located between SL1 and the first codons of the gag open reading frame.
- Binding of antisense oligonucleotides directed against the 5' side of stem B and nucleotides upstream increased the tight dimerization rate for all three lentiviral RNAs tested.
Takeaway
This study found that different types of viruses can control how their RNA sticks together, which is important for making new viruses.
Methodology
The study compared the dimerization properties of SIVmac239, HIV-1, and HIV-2 leader RNA fragments using homologous constructs and experimental conditions.
Digital Object Identifier (DOI)
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