Side population rather than CD133+ cells distinguishes enriched tumorigenicity in hTERT-immortalized primary prostate cancer cells
2011

Side Population Cells Show Higher Tumorigenicity in Prostate Cancer

Sample size: 6 publication 10 minutes Evidence: moderate

Author Information

Author(s): Zhou Jianjun, Wang Honghe, Cannon Virginetta, Wolcott Karen Marie, Song Hongbin, Yates Clayton

Primary Institution: Tuskegee University

Hypothesis

Does the side population (SP) of prostate cancer cells have a higher tumorigenic potential compared to CD133+ cells?

Conclusion

The study found that side population cells, rather than CD133+ cells, are enriched with tumorigenic stem-like cells capable of generating prostate tumors.

Supporting Evidence

  • CD133+ and CD133- cells exhibited similar tumorigenicity in vitro and in vivo.
  • SP cells showed an 8-fold enhanced tumorigenic potential compared to CD133+ cells.
  • FACS-sorted CD133+ cells represented less than 0.1% of the total cell population.

Takeaway

Some cancer cells can act like stem cells and make tumors. This study found that a special group of these cells, called side population cells, are better at making tumors than another group called CD133+ cells.

Methodology

The study used in vitro assays, flow cytometry, and magnetic cell sorting to analyze the tumorigenic potential of side population and CD133+ cells in prostate cancer.

Limitations

The study primarily focused on hTERT-immortalized prostate cancer cell lines, which may not fully represent the complexity of primary tumors.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1476-4598-10-112

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