Side Population Cells Show Higher Tumorigenicity in Prostate Cancer
Author Information
Author(s): Zhou Jianjun, Wang Honghe, Cannon Virginetta, Wolcott Karen Marie, Song Hongbin, Yates Clayton
Primary Institution: Tuskegee University
Hypothesis
Does the side population (SP) of prostate cancer cells have a higher tumorigenic potential compared to CD133+ cells?
Conclusion
The study found that side population cells, rather than CD133+ cells, are enriched with tumorigenic stem-like cells capable of generating prostate tumors.
Supporting Evidence
- CD133+ and CD133- cells exhibited similar tumorigenicity in vitro and in vivo.
- SP cells showed an 8-fold enhanced tumorigenic potential compared to CD133+ cells.
- FACS-sorted CD133+ cells represented less than 0.1% of the total cell population.
Takeaway
Some cancer cells can act like stem cells and make tumors. This study found that a special group of these cells, called side population cells, are better at making tumors than another group called CD133+ cells.
Methodology
The study used in vitro assays, flow cytometry, and magnetic cell sorting to analyze the tumorigenic potential of side population and CD133+ cells in prostate cancer.
Limitations
The study primarily focused on hTERT-immortalized prostate cancer cell lines, which may not fully represent the complexity of primary tumors.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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