Comparing Two Methods for Measuring IGF-1 in Breast Cancer
Author Information
Author(s): Jones Laundette P, Stefansson Steingrimur, Kim Man S, Ahn Saeyoung N
Primary Institution: University of Maryland School of Medicine
Hypothesis
Can carbon nanotube field-effect transistors (CNT-FET) provide a simpler and faster method for measuring IGF-1 compared to traditional radioimmunoassays?
Conclusion
The CNT-FET assay is simpler and faster than the radioimmunoassay while providing comparable results.
Supporting Evidence
- CNT-FETs required only one antibody and provided real-time results.
- CNT-FETs used approximately 100-fold less serum than radioimmunoassays.
- Both assays showed a statistically significant increase in serum IGF-1 levels in Brca1f/f; MMTV-Cre mice.
Takeaway
This study shows that a new test using tiny tubes can measure a cancer-related protein faster and with less blood than the old method.
Methodology
The study compared CNT-FET and radioimmunoassay methods for measuring serum IGF-1 levels in a mouse model.
Limitations
The study was conducted in a mouse model, which may not fully represent human conditions.
Participant Demographics
Brca1 conditional knockout mice and wild type C57Bl/6 mice were used.
Statistical Information
P-Value
p ≤ 0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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