DNA Ploidy and S-Phase Fraction in Bladder Cancer
Author Information
Author(s): S.D. Fossa, A.A. Berner, A.-B. Jacobsen, H. Wehre, B. Kvarstein, T. Urnes, P. Ogreid, T.E. Bjerklund Johansen, J. Silde, J.M. Nesland, E.O. Pettersen
Primary Institution: The Norwegian Radium Hospital
Hypothesis
What is the prognostic significance of DNA ploidy and S-phase fraction in muscle-invasive bladder cancer?
Conclusion
DNA ploidy does not represent a prognostic parameter in muscle-invasive operable bladder carcinomas, but a high S-phase fraction may help identify patients with chemotherapy-sensitive tumors.
Supporting Evidence
- Patients with high S-phase fraction tumors tended to have a better prognosis than those with low S-phase fraction tumors.
- 51% of tumors were p53 positive, with a higher occurrence in low S-phase fraction tumors.
- Chemotherapy improved the 5-year survival rate for patients with high S-phase fraction tumors.
Takeaway
This study looked at how the DNA of bladder cancer cells can help doctors understand how serious the cancer is and how well it might respond to treatment. They found that some DNA features are not helpful for predicting outcomes.
Methodology
The study analyzed DNA ploidy and S-phase fraction in 118 patients using flow cytometry and correlated these findings with immunohistochemical markers.
Limitations
The study may have inter-laboratory variability in DNA flow cytometric analysis.
Participant Demographics
The median age of participants was 66 years, with 90 males and 28 females.
Statistical Information
P-Value
P<0.05 for high SPF tumors with chemotherapy
Statistical Significance
p<0.05
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