Rapid Test for Diagnosing Shigella dysenteriae 1
Author Information
Author(s): Taneja Neelam, Nato Faridabano, Dartevelle Sylvie, Sire Jean Marie, Garin Benoit, Thi Phuong Lan Nguyen, Diep Tai The, Shako Jean Christophe, Bimet François, Filliol Ingrid, Muyembe Jean-Jacques, Ungeheuer Marie Noëlle, Ottone Catherine, Sansonetti Philippe, Germani Yves
Primary Institution: Postgraduate Institute of Medical Education and Research, Chandigarh, India
Hypothesis
Can a dipstick test effectively detect Shigella dysenteriae 1 in stool samples?
Conclusion
The dipstick test shows promising potential for rapid diagnosis of Shigella dysenteriae 1.
Supporting Evidence
- The test detected S. dysenteriae 1 in 10 minutes.
- Specificity was 98.7% in clinical samples.
- Sensitivity was 91.7% in the study.
- Concordance with stool cultures was 98.4%.
- Positive predictive value was 73.3%.
- Negative predictive value was 99.7%.
- The test can be performed by minimally trained personnel.
Takeaway
Researchers created a quick test to find a germ that causes diarrhea, which can help doctors treat sick people faster.
Methodology
The test detects S. dysenteriae 1 lipopolysaccharide using monoclonal antibodies on a dipstick.
Potential Biases
Potential for misinterpretation of faint test lines as positive results.
Limitations
The study's sensitivity may be affected by low concentrations of the pathogen in stool samples.
Participant Demographics
Participants included individuals from India, Vietnam, Senegal, France, and the Democratic Republic of Congo.
Statistical Information
P-Value
0.0001
Confidence Interval
95% CI: 96.6–99.6%
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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