Interactions Between Platelets and Leukocytes After Activation by LPS
Author Information
Author(s): Xiong Jing, Miller Virginia M., Hunter Larry W., Li Yunman, Jayachandran Muthuvel
Primary Institution: Mayo Clinic
Hypothesis
Acute exposure to a sentinel dose of LPS would induce microvesicle production and exchange of specific proteins/receptors between platelets and leukocytes via TLR4 activation.
Conclusion
Leukocytes exchange surface antigens with platelets through TLR4 activation within one hour of exposure to LPS.
Supporting Evidence
- Platelets from wild type mice showed increased positivity for leukocyte antigens after LPS exposure.
- Leukocyte antigen expression on platelets was not observed in blood from TLR4 gene deleted mice.
- Seven days after LPS injection, leukocytes retained platelet antigens.
Takeaway
When bacteria trigger an immune response, platelets and white blood cells can swap markers on their surfaces, which might affect how our blood clots.
Methodology
Blood from wild type and TLR4 gene deleted mice was incubated with LPS, and interactions were analyzed using flow cytometry.
Limitations
The study did not determine the half-life of platelet-leukocyte aggregates.
Participant Demographics
Male and female wild type and TLR4 gene deleted mice aged four to eight months.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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