BCL9's Role in Wnt Signaling and Colorectal Cancer
Author Information
Author(s): de la Roche Marc, Worm Jesper, Bienz Mariann
Primary Institution: MRC Laboratory of Molecular Biology
Hypothesis
BCL9 is required for efficient β-catenin-mediated transcription in colorectal cancer cells.
Conclusion
BCL9 is essential for effective β-catenin-mediated transcription in colorectal cancer cells, suggesting it could be a target for cancer treatment.
Supporting Evidence
- BCL9 is required for efficient β-catenin-mediated transcription in Wnt-stimulated HEK 293 cells.
- BCL9 and B9L are both Wnt-inducible genes, hyperexpressed in colorectal cancer cell lines.
- Depletion of BCL9 reduces the expression of Wnt target genes like c-myc and AXIN2.
Takeaway
BCL9 helps a protein called β-catenin work properly in cells that can lead to colon cancer, which means it might be a good target for new medicines.
Methodology
The study used overexpression and RNAi-mediated depletion of BCL9 in human cell lines to assess its function in Wnt signaling.
Limitations
The study primarily focused on specific cell lines and may not fully represent all colorectal cancer types.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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