Mapping Plasmid Transcriptomes in Bacteria
Author Information
Author(s): Miyakoshi Masatoshi, Nishida Hiromi, Shintani Masaki, Yamane Hisakazu, Nojiri Hideaki
Primary Institution: The University of Tokyo
Hypothesis
The expression range of plasmid genes that will result in phenotypic variation has not been quantitatively investigated.
Conclusion
The study shows that the levels of transcription for the operons on a plasmid can vary by host background.
Supporting Evidence
- The transcription patterns of plasmid genes can change based on the host bacteria.
- High-resolution mapping using tiling arrays allows for detailed analysis of prokaryotic transcriptomes.
- The study identified 49 transcription units on the plasmid pCAR1.
Takeaway
This study looked at how genes on a plasmid behave differently depending on the bacteria they are in, showing that the environment can change how these genes work.
Methodology
Used a microarray with evenly tiled probes at a density of 9 bp to map and quantify transcripts of the plasmid pCAR1 in different bacterial hosts.
Limitations
The study did not address whether and how the plasmid transcriptome in the original host differed from that in the transconjugant.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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