Betulinic Acid Increases Cytotoxicity and Radiosensitivity in Glioma Cells Under Low Oxygen
Author Information
Author(s): Bache Matthias, Zschornak Martin P, Passin Sarina, Keßler Jacqueline, Wichmann Henri, Kappler Matthias, Paschke Reinhard, Kaluđerović Goran N, Kommera Harish, Taubert Helge, Vordermark Dirk
Primary Institution: Martin-Luther-University Halle-Wittenberg
Hypothesis
The combination of betulinic acid with radiotherapy will enhance the cytotoxic effects in glioma cells under hypoxic conditions.
Conclusion
Betulinic acid improves the effects of tumor therapy in glioma cells, especially under hypoxic conditions.
Supporting Evidence
- Betulinic acid showed increased cytotoxicity in glioma cells under hypoxic conditions.
- The combination of betulinic acid with radiotherapy resulted in an additive effect.
- Under normoxic conditions, the IC50 was 23 μM for U251MG and 24 μM for U343MG cells.
- Betulinic acid treatment led to decreased cell migration and increased apoptosis.
- Radiation enhancement factors were observed at doses of 15 Gy after treatment with betulinic acid.
Takeaway
Betulinic acid helps kill cancer cells better when there's less oxygen, making it a good partner for radiation therapy.
Methodology
The study analyzed the effects of betulinic acid on glioma cell lines using clonogenic survival assays, migration assays, and Western blot analyses.
Limitations
The study did not explore the long-term effects of betulinic acid or its mechanisms in detail.
Statistical Information
P-Value
p = 0.004, p = 0.01, p = 0.02, p = 0.03
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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