Protein Expression in Malaria Parasites
Author Information
Author(s): Bernardo J. Foth, Neng Zhang, Sachel Mok, Peter R. Preiser, Zbynek Bozdech
Primary Institution: School of Biological Sciences, Nanyang Technological University, Singapore
Hypothesis
The study investigates the extent of post-transcriptional regulation and post-translational modifications in schizont-stage malaria parasites.
Conclusion
The study concludes that both post-transcriptional and post-translational events are widespread and significant during the intra-erythrocytic development of Plasmodium falciparum.
Supporting Evidence
- More than half of all protein spots exhibit a statistically significant change in protein abundance across the four time points.
- 278 proteins/isoforms showed a fold change in excess of 1.4×.
- 54 protein spots were identified by mass spectrometry, revealing multiple isoforms for many proteins.
Takeaway
This study looks at how malaria parasites change their proteins as they grow, showing that many proteins can change a lot during their life cycle.
Methodology
The study used two-dimensional differential gel electrophoresis (2D-DIGE) to analyze protein abundance in malaria parasites at different time points.
Limitations
The study primarily focused on a limited time frame of the intra-erythrocytic developmental cycle and may not capture all protein dynamics.
Statistical Information
P-Value
<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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