The Histone Demethylase Jarid1b (Kdm5b) Is a Novel Component of the Rb Pathway and Associates with E2f-Target Genes in MEFs during Senescence
2011

Jarid1b's Role in the Rb Pathway and E2f-Target Genes During Senescence

publication Evidence: moderate

Author Information

Author(s): Nijwening Jeroen H., Geutjes Ernst-Jan, Bernards Rene, Beijersbergen Roderick L.

Primary Institution: The Netherlands Cancer Institute, Amsterdam, The Netherlands

Hypothesis

Jarid1b is a critical component of the Rb pathway that associates with E2f-target genes during cellular senescence.

Conclusion

Jarid1b functions in the Rb pathway to repress E2f-target genes during senescence, and its knockdown can bypass cellular senescence.

Supporting Evidence

  • Jarid1b associates with E2f-target genes during senescence.
  • Knockdown of Jarid1b prevents senescence in murine cells.
  • Jarid1b knockdown restores expression of cell cycle genes.
  • Jarid1b is involved in Rb-mediated repression of E2f-target genes.

Takeaway

Jarid1b helps control when cells stop dividing, and if you take it away, the cells can keep growing even when they should stop.

Methodology

Functional genetic screening model in murine cells to identify chromatin remodeling enzymes involved in the Rb pathway.

Digital Object Identifier (DOI)

10.1371/journal.pone.0025235

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