A BAC end view of the Musa acuminata genome
2007
Understanding the Musa acuminata Genome
Sample size: 6252
publication
Evidence: moderate
Author Information
Author(s): Cheung Foo, Town Christopher D
Primary Institution: J Craig Venter Institute
Hypothesis
Can BAC end sequencing provide insights into the genome composition of Musa acuminata?
Conclusion
The study suggests that BAC end sequencing can help anchor parts of the Musa acuminata genome to the genomes of Oryza sativa and Arabidopsis.
Supporting Evidence
- The BAC end-sequences were shown to contain homology to proteins, expressed sequence tags, transposons, and repeat sequences.
- Approximately 600 BAC end-sequences contained protein sequences not found in existing databases.
- A total of 352 potential SSR markers were discovered in the BAC end-sequences.
Takeaway
Scientists looked at the DNA of bananas to learn more about their genes and how they relate to other plants like rice.
Methodology
BAC end sequencing was performed on a library constructed from leaves of the wild diploid 'Calcutta 4' clone.
Limitations
The BAC end sequencing approach may not provide a truly random sampling of the genome.
Digital Object Identifier (DOI)
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