Unconventional Repertoire Profile Is Imprinted during Acute Chikungunya Infection for Natural Killer Cells Polarization toward Cytotoxicity Clonal Expansion of NK Cells in CHIKV Infection
2011

Chikungunya Virus Infection and Natural Killer Cells

Sample size: 25 publication 10 minutes Evidence: high

Author Information

Author(s): Caroline Petitdemange, Pierre Becquart, Nadia Wauquier, Vivien Béziat, Patrice Debré, Eric M. Leroy, Vincent Vieillard

Primary Institution: INSERM UMR-S 945, Immunité et Infection, Hôpital Pitié-Salpêtrière, Paris, France

Hypothesis

The study aims to analyze the phenotypic and functional characteristics of NK cells during acute Chikungunya virus infection.

Conclusion

Chikungunya virus infection leads to a transient expansion of a unique subset of NK cells that are associated with viral load and exhibit significant cytolytic activity.

Supporting Evidence

  • NK cells from CHIKV-infected patients showed a significant increase in frequency compared to healthy controls.
  • The expansion of NK cells was correlated with the viral load in patients.
  • Functional tests indicated that NK cells had enhanced cytolytic activity against infected cells.
  • Chikungunya virus infection was associated with a transient modulation of NK cell receptor expression.

Takeaway

When people get sick from Chikungunya virus, their immune cells called NK cells grow in number and help fight the virus, but they also have trouble making a special signal that helps other immune cells.

Methodology

Flow cytometry was used to assess the frequency and activation status of NK cells in CHIKV-infected patients compared to healthy controls.

Potential Biases

Potential bias due to the limited geographic and demographic diversity of the study participants.

Limitations

The study primarily focuses on a specific population and may not be generalizable to all demographics.

Participant Demographics

25 CHIKV-infected patients (mean age 32±15 years, 60% women) from Gabon.

Statistical Information

P-Value

p<0.0001

Statistical Significance

p<0.0001

Digital Object Identifier (DOI)

10.1371/journal.ppat.1002268

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