The Role of Platelet GPIbα in Ischemic Stroke in Mice
Author Information
Author(s): Simon F De Meyer, Tobias Schwarz, Daphne Schatzberg, Denisa D Wagner
Primary Institution: Immune Disease Institute, Boston, USA
Hypothesis
Does the glycoprotein Ibα play a critical role in ischemic stroke outcomes in mice?
Conclusion
The study shows that mice lacking the extracellular part of GPIbα have significantly smaller infarct volumes and better functional outcomes after ischemic stroke.
Supporting Evidence
- GPIbα/IL4Rα mice had a 50% reduction in infarct size compared to WT mice.
- Functional outcomes were significantly better in GPIbα/IL4Rα mice as measured by the Bederson score.
- No major anatomical differences in cerebral vasculature were observed between GPIbα/IL4Rα and WT mice.
Takeaway
This study found that a special protein on platelets, called GPIbα, is really important for how bad a stroke can be in mice. If this protein is missing, the mice do much better after a stroke.
Methodology
The study used transgenic mice with modified GPIbα and compared their stroke outcomes to wild-type mice after inducing ischemia.
Potential Biases
Potential bias due to the use of a specific mouse model and the exclusion of animals that died shortly after the procedure.
Limitations
The study was conducted in mice, which may not fully represent human stroke conditions.
Participant Demographics
8-10 weeks old male mice on a C57BL/6J background.
Statistical Information
P-Value
p < 0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website