Ndt80's Role in Fluconazole Resistance in Candida albicans
Author Information
Author(s): Christoph Sasse, Rebecca Schillig, Franziska Dierolf, Michael Weyler, Sabrina Schneider, Selene Mogavero, P. David Rogers, Joachim Morschhäuser
Primary Institution: Institut für Molekulare Infektionsbiologie, Universität Würzburg, Würzburg, Germany
Hypothesis
Is Ndt80 required for the induction of MDR1 and CDR2 expression in Candida albicans?
Conclusion
Ndt80 is not necessary for the overexpression of key genes involved in fluconazole resistance in Candida albicans.
Supporting Evidence
- Ndt80 is not required for the induction of the MDR1 promoter by benomyl.
- Ndt80 has a negative effect on Mrr1-mediated fluconazole resistance.
- Ndt80 is a repressor of CDR2 expression under noninducing conditions.
- Hyperactive forms of Mrr1, Tac1, and Upc2 can still induce their target genes without Ndt80.
Takeaway
Ndt80 is a protein in yeast that helps control how the yeast responds to antifungal drugs, but it turns out that it's not needed for the yeast to become resistant to these drugs.
Methodology
The study involved creating mutants of Candida albicans and analyzing their gene expression and drug resistance through various assays.
Limitations
The study may not account for all environmental conditions affecting Ndt80's role in drug resistance.
Digital Object Identifier (DOI)
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