MicroRNA Changes in HIV-1 Positive Individuals
Author Information
Author(s): Houzet Laurent, Yeung Man Lung, de Lame Valery, Desai Dhara, Smith Stephen M, Jeang Kuan-Teh
Primary Institution: National Institute of Allergy and Infectious Diseases, National Institutes of Health
Hypothesis
How do human microRNA patterns vary at various stages of HIV-1 infection?
Conclusion
Specific microRNA signatures can be observed for different classes of HIV-1 seropositive individuals.
Supporting Evidence
- 59 out of 62 frequently-changed miRNAs were down regulated in HIV-1 positive individuals.
- Specific miRNA signatures were identified for each of the four classes of HIV-1 seropositive individuals.
- Changes in miRNA profiles were more closely modeled by T-cell activation than by inactivation.
Takeaway
This study looked at tiny molecules called microRNAs in people with HIV, finding that these molecules change in different ways depending on the stage of the infection.
Methodology
MicroRNA expression was profiled in peripheral blood mononuclear cells from 36 HIV-1 seropositive individuals and 12 normal controls using microarrays.
Limitations
The study did not fractionate PBMC samples into cellular subsets, which may affect the interpretation of miRNA signatures.
Participant Demographics
36 HIV-1 seropositive individuals categorized into four classes based on CD4+ T-cell counts and viral loads.
Digital Object Identifier (DOI)
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