MCRS2 represses the transactivation activities of Nrf1
2009
MCRS2 and its Role in Repressing Nrf1 Activity
publication
Evidence: moderate
Author Information
Author(s): Wu Jia-Long, Lin Young-Sun, Yang Chi-Chiang, Lin Yu-Jen, Wu Shan-Fu, Lin Ying-Ting, Huang Chien-Fu
Primary Institution: IBMS, Academia Sinica, Taipei, Taiwan
Hypothesis
MCRS2 functions as a repressor of Nrf1-mediated transcriptional activation.
Conclusion
MCRS2 has been identified as a new repressor protein that negatively regulates Nrf1-mediated transcriptional activation.
Supporting Evidence
- MCRS2 was isolated as an Nrf1-interacting partner through yeast two-hybrid screening.
- The interaction between Nrf1 and MCRS2 was confirmed using GST pull-down assays.
- MCRS2 was shown to colocalize with Nrf1 in the nucleus.
- MCRS2 repressed transcriptional activity driven by Nrf1 in reporter assays.
Takeaway
MCRS2 is like a stop sign for a traffic light, telling Nrf1 to slow down and not activate certain genes.
Methodology
Yeast two-hybrid screening, GST pull-down assays, and co-immunoprecipitation were used to study the interaction between MCRS2 and Nrf1.
Digital Object Identifier (DOI)
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