Dephosphorylated NSSR1 Is Induced by Androgen in Mouse Epididymis and Phosphorylated NSSR1 Is Increased during Sperm Maturation
2011
NSSR1 and Its Role in Sperm Maturation
Sample size: 9
publication
Evidence: moderate
Author Information
Author(s): Xiao Ping-Jie, Peng Zheng-Yu, Huang Lu, Li Ya, Chen Xian-Hua
Primary Institution: Fudan University, Shanghai, China
Hypothesis
The expression of NSSR1 in mouse epididymis is regulated by hormones such as androgen and estradiol.
Conclusion
NSSR1 is hormonally regulated and plays a significant role in sperm maturation and fertilization.
Supporting Evidence
- NSSR1 expression increased significantly during the development of the caput epididymis.
- Phosphorylated NSSR1 was mainly expressed in the apical side of epithelial cells.
- Dephosphorylated NSSR1 was upregulated in a testosterone-dependent manner.
- NSSR1 was detected in the mid-piece of the sperm tail and the acrosome.
Takeaway
NSSR1 is a protein that helps sperm grow up and get ready to fertilize an egg, and its levels change with hormones.
Methodology
The study involved Western blot analysis and immunofluorescent staining to examine NSSR1 expression in mouse epididymis and sperm.
Participant Demographics
Adult male mice were used in the study.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website