LITAF's Role in TNF-α Secretion from Inflamed Colonic Macrophages
Author Information
Author(s): Kristen N. Bushell, Susan E. Leeman, Earl Gillespie, Adam C. Gower, Karen L. Reed, Arthur F. Stucchi, James M. Becker, Salomon Amar
Primary Institution: Boston University
Hypothesis
Does LITAF mediate TNF-α expression in acutely inflamed colonic tissues?
Conclusion
LITAF contributes to the regulation of TNF-α secretion in macrophages from inflamed colonic tissue.
Supporting Evidence
- LITAF mRNA and protein levels were elevated in macrophages from inflamed tissues.
- Macrophages from LITAF knockout mice secreted less TNF-α in response to LPS.
- TNF-α secretion was significantly increased in macrophages from inflamed colonic tissue compared to non-inflamed tissue.
Takeaway
This study shows that a protein called LITAF helps control a substance that causes inflammation in the gut, which could help in treating diseases like Crohn's.
Methodology
The study used a mouse model of colonic inflammation to analyze TNF-α secretion from macrophages harvested from inflamed and non-inflamed tissues.
Limitations
The study primarily focused on a specific mouse strain, which may limit the generalizability of the findings.
Participant Demographics
Adult, gender-matched male and female C57BL/6 mice.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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