Brimonidine's Effect on Nitric Oxide Synthase in Human Ciliary Bodies
Author Information
Author(s): Wu Renyi, Yin Jinfu, Yao Ke, Haefliger Ivan
Primary Institution: Eye Center of the 2nd Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, China
Hypothesis
Does brimonidine inhibit the expression of nitric oxide synthase (NOS) induced by forskolin in human ciliary bodies?
Conclusion
Brimonidine inhibits the up-regulation of neuronal NOS expression induced by forskolin in human ciliary bodies.
Supporting Evidence
- Forskolin significantly up-regulated the mRNA and protein expression of nNOS.
- Brimonidine significantly inhibited the forskolin-induced increase in nNOS expression.
- Three NOS isoforms were detected in human ciliary bodies.
Takeaway
This study found that a drug called brimonidine can stop another substance, forskolin, from increasing a specific protein that helps make a chemical called nitric oxide in the eye.
Methodology
Human ciliary bodies were incubated with forskolin and brimonidine, and the expression of NOS isoforms was assessed using RT-PCR and Western blot analysis.
Limitations
The study used frozen-thawed tissues, which may have influenced the results.
Participant Demographics
Human ciliary bodies obtained from eye bank after corneal transplantation.
Statistical Information
P-Value
p<0.001 for forskolin, p<0.01 for brimonidine
Statistical Significance
p<0.05
Want to read the original?
Access the complete publication on the publisher's website