Neuraminidase Inhibitor Susceptibility Testing in Human Influenza Viruses
Author Information
Author(s): Okomo-Adhiambo Margaret, Sleeman Katrina, Ballenger Kristina, Nguyen Ha T., Mishin Vasiliy P., Sheu Tiffany G., Smagala James, Li Yan, Klimov Alexander I., Gubareva Larisa V.
Primary Institution: Centers for Disease Control and Prevention
Hypothesis
This study aims to assess the susceptibility of influenza viruses to neuraminidase inhibitors during the 2008-2009 season.
Conclusion
The study found that a high percentage of seasonal H1N1 viruses were resistant to oseltamivir, while pandemic H1N1 viruses showed much lower resistance.
Supporting Evidence
- 93.3% of seasonal H1N1 viruses tested were found to be oseltamivir-resistant.
- Only 0.7% of pandemic H1N1 viruses tested were resistant to oseltamivir.
- All influenza A(H3N2) and B viruses tested were sensitive to oseltamivir, except for a few outliers.
- All viruses tested were sensitive to zanamivir, with some exceptions among seasonal A(H1N1) and A(H3N2) variants.
Takeaway
The study looked at how well different flu viruses respond to certain medicines, finding that many seasonal flu viruses didn't work well with one of the main treatments.
Methodology
The study used chemiluminescent neuraminidase inhibition assays to test virus isolates for susceptibility to neuraminidase inhibitors.
Potential Biases
Potential bias may arise from the use of cell culture methods that could select for certain virus variants.
Limitations
The study's findings may not fully represent the clinical effectiveness of neuraminidase inhibitors due to the lack of direct correlation with drug concentrations needed to inhibit virus replication in humans.
Digital Object Identifier (DOI)
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