Versican 3'UTR Induces Organ Adhesion by Regulating miR-199a* Functions
Author Information
Author(s): Lee Daniel Y., Shatseva Tatiana, Jeyapalan Zina, Du William W., Deng Zhaoqun, Yang Burton B.
Primary Institution: Sunnybrook Research Institute, Sunnybrook Health Sciences Centre, Toronto, Canada
Hypothesis
The non-coding 3'UTR plays a role in the regulation of miRNA function.
Conclusion
The expression of versican 3'UTR induces organ adhesion in transgenic mice by modulating miR-199a* activities.
Supporting Evidence
- Transgenic mice expressing versican 3'UTR showed enhanced organ adhesion.
- Expression of versican and fibronectin was up-regulated in transgenic mice.
- Luciferase assays confirmed the binding of miR-199a* to the versican 3'UTR.
- Cell adhesion assays indicated that down-regulation of the 3'UTR increased cell adhesion.
- Histological analysis showed increased versican expression in various organs of transgenic mice.
Takeaway
This study shows that a part of a gene called 3'UTR can help organs stick together by changing how a tiny molecule called miRNA works.
Methodology
Transgenic mice expressing versican 3'UTR were created, and various assays including PCR, RT-PCR, and luciferase assays were performed to analyze gene expression and organ adhesion.
Limitations
The study primarily focuses on a specific miRNA and its interaction with the 3'UTR, which may not represent all possible interactions.
Participant Demographics
Transgenic mice were used in the study.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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