Isolation and characterization of an exopolygalacturonase from Fusarium oxysporum f.sp. cubense race 1 and race 4
2011
Study of an Enzyme from a Banana Pathogen
publication
Evidence: moderate
Author Information
Author(s): Dong Zhangyong, Wang Zhenzhong
Primary Institution: South China Agricultural University
Hypothesis
Can exopolygalacturonase from Fusarium oxysporum f.sp. cubense race 4 be isolated and characterized?
Conclusion
The study successfully isolated and characterized an exopolygalacturonase from Fusarium oxysporum f.sp. cubense race 4, demonstrating its potential role in banana pathogenicity.
Supporting Evidence
- The enzyme PGC2 was isolated and purified from Fusarium oxysporum f.sp. cubense race 4.
- PGC2 showed optimal activity at 50°C and pH 5.
- Both recombinant PGC2 proteins could induce tissue maceration and necrosis in banana plants.
Takeaway
Scientists found an enzyme from a fungus that can hurt banana plants, which helps us understand how this fungus causes disease.
Methodology
The enzyme was isolated from the pathogen's culture, purified, and characterized using various biochemical techniques.
Digital Object Identifier (DOI)
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