How the Glucocorticoid Receptor Controls Gene Activity
Author Information
Author(s): So Alex Yick-Lun, Chaivorapol Christina, Bolton Eric C, Li Hao, Yamamoto Keith R
Primary Institution: University of California San Francisco
Hypothesis
Is GR occupancy a limiting factor for glucocorticoid response in A549 cells?
Conclusion
The study concludes that GR occupancy is a primary determinant of glucocorticoid responsiveness in A549 cells.
Supporting Evidence
- GR occupancy was found to be predominantly near glucocorticoid-responsive genes in A549 cells.
- 63% of GREs were located more than 10 kb from transcription start sites.
- The core GR binding sequences varied widely but were conserved at individual GREs across species.
Takeaway
The glucocorticoid receptor helps control which genes are active in a cell, and it does this in a way that can be different for each type of cell.
Methodology
The study used chromatin immunoprecipitation-microarray (ChIP-chip) to identify GR binding regions in A549 cells.
Limitations
The study primarily focuses on A549 cells, which may not represent GR function in other cell types.
Digital Object Identifier (DOI)
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